ZEISS and JoVE present open access video publications with stereo, zoom, and light sheet fluorescence microscopy

The Journal of Visualized Experiments (JoVE) is a PubMed-indexed video journal with the mission to increase the productivity of scientific research. Our article features two most recent video protocols for Zebrafish imaging with ZEISS with stereo, zoom, and light sheet fluorescence microscopy systems. Enjoy the video protocols by our partners, and let yourself be inspired!

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Jaroslav Icha*¹, Christopher Schmied*¹, Jaydeep Sidhaye¹, Pavel Tomancak¹, Stephan Preibisch^1,2,3, Caren Norden¹

¹Max Planck Institute of Molecular Cell Biology and Genetics, ²HHMI Janelia Research Campus, ³Berlin Institute of Medical Systems Biology of the Max Delbrück Center

* These authors contributed equally

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets. Light sheet fluorescence microscopy was performed with ZEISS Lightsheet Z.1.

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Birgit Perner¹, Danny Schnerwitzki¹, Michael Graf^1,3, Christoph Englert^1,2

¹Molecular Genetics, Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), ²Faculty of Biology and Pharmacy, Friedrich Schiller University of Jena, ³Carl Zeiss Microscopy GmbH

The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift. ZEISS instruments used include stereo & zoom microscopes, Axiocam, and ApoTome.

More open access JoVE publications with ZEISS microscopes:

• Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
• Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
• Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device

About JoVE

JoVE, the Journal of Visualized Experiments, is the world’s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.

All JoVE articles are indexed in subject-relevant indexing sites, including PubMed/MEDLINE, SciFinder and Scopus. Publishing in JoVE allows authors to dynamically present their methods, data analyses and results clearly, accurately, and professionally with the guidance of JoVE’s professional videographers and editors. JoVE has published thousands of video articles from top research institutions around the world.

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